学术探索
学术期刊
新闻热点
数据分析
智能评审

HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF BIOGENIC POLYAMINES USING 2-(1-PYRENYL)ETHYL CHLOROFORMATE AS A NEW FLUOROGENIC DERIVATIZING REAGENT

被引：19
作者：
CICHY, MA
STEGMEIER, DL
VEENING, H
BECKER, HD
机构：
[1] BUCKNELL UNIV,DEPT CHEM,LEWISBURG,PA 17837
[2] UNIV GOTHENBURG,S-41296 GOTHENBURG,SWEDEN
[3] CHALMERS UNIV TECHNOL,DEPT ORGAN CHEM,S-41296 GOTHENBURG,SWEDEN
来源：
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 613卷 / 01期
关键词：
D O I：
10.1016/0378-4347(93)80192-7
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The application of a new fluorogenic pre-column derivatizing reagent, 2-(1-pyrenyl)ethyl chloroformate (PEOC), is reported for the separation and detection of biogenic polyamines using column liquid chromatography. The development of the method included the optimization of excitation and emission wavelengths, efficient gradient programming, derivatization temperature, time, and pH. Minimum detection limits, linear ranges, reproducibility, and recovery from analyzed samples were determined. The procedure was applied to hydrolyzed serum samples taken from healthy individuals and cancer patients. Separation of PEOC-derivatized polyamines from the serum hydrolysis by-products was successful and detection limits were more favorable than those previously reported for 9-fluorenylmethyl chloroformate-derivatized polyamines.
引用
收藏
页码：15 / 21
页数：7
相关论文
共 20 条
12