THE ROLE OF THE CRABTREE EFFECT AND AN ENDOGENOUS FUEL IN THE ENERGY-METABOLISM OF RESTING AND PROLIFERATING THYMOCYTES

被引:161
作者
GUPPY, M [1 ]
GREINER, E [1 ]
BRAND, K [1 ]
机构
[1] UNIV ERLANGEN NURNBERG,FAC MED,DEPT BIOCHEM,W-8520 ERLANGEN,GERMANY
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1993年 / 212卷 / 01期
关键词
D O I
10.1111/j.1432-1033.1993.tb17637.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Rat thymocytes have been used to characterize the changes in energy metabolism that occur as cells undergo a resting/proliferation transition. In the resting state, anaerobic ATP production accounts for only 4% of ATP turnover. The remainder is fueled by the oxidation of a mixture of an unidentified endogenous fuel (62%), glucose (18%) and glutamine (16%). 48 h after mitogen stimulation, the ATP turnover has increased twofold. In these proliferating cells, glucose inhibits oxygen consumption by 58%, indicating a profound Crabtree effect which is not present in resting cells. Consequently, proliferating cells, in the presence of glucose and glutamine, fuel the majority (61%) of ATP turnover anaerobically, producing lactate from glucose. The development of a Crabtree effect may be the result of the 8-10-fold increase in glycolytic enzyme activities which occurs with proliferation. Possible advantages of such a proliferative metabolism are a sparing of endogenous fuel, and a minimizing of oxidative metabolism, with its concurrent production of free radicals.
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页码:95 / 99
页数:5
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