DETERMINATION OF ETHYNYLESTRADIOL AND NORETHINDRONEIN A SINGLE SPECIMEN OF PLASMA BY AUTOMATED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND SUBSEQUENT RADIOIMMUNOASSAY

被引：10

作者：

LEE, GJL

OYANG, MH

BAUTISTA, J

KUSHINSKY, S

机构：

[1] Department of Analytical and Metabolic Chemistry, Syntex Research, Palo Alto, CA 94304, United States

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1987年 / 10卷 / 10期

关键词：

CHROMATOGRAPHIC ANALYSIS - Liquid - DRUG PRODUCTS - Chromatographic Analysis;

D O I：

10.1080/01483918708068913

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described for the specific determination of ethinylestradiol and norethindrone in the same specimen of plasma at concentrations of 20 and 50 pg/ml, respectively. The method is based on fully automated high-performance liquid chromatography for separation of the analytes from one another and from coextracted extraneous components and on radioimmunoassay using antisera of high specificity for the quantification. Validation of the method included demonstration of low procedural blanks, recoverability of added hormones and low intra-assay and inter-assay variability.

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页码：2305 / 2318

页数：14

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