Glucose oxidase is immobilized onto a cellulose acetate membrane by glutaraldehyde linkage, and the membrane is used to cover the platinum electrode of a hydrogen peroxide sensor. A silanized polycarbonate membrane then covers the enzyme layer, and extends the linear calibration range to higher concentrations. The sensor, when incorporated into a flow-injection system, allows the determination of glucose at levels up to 1 M in soft drinks at a rate of 60 samples h-1 without sample dilution.