MEASUREMENT OF S-PHASE DURATION IN HUMAN EPIDERMIS USING CYCLIN IMMUNOSTAINING AND H-3 THYMIDINE PULSE LABELING

被引：8

作者：

BERLINGIN, E

HEENEN, M

GALAND, P

机构：

[1] FREE UNIV BRUSSELS,HOSP ERASME,DEPT DERMATOL,B-1070 BRUSSELS,BELGIUM

[2] FREE UNIV BRUSSELS,SCH MED,INST INTERDISCIPLINARY RES,CYTOL & EXPTL CANCEROL LAB,B-1050 BRUSSELS,BELGIUM

[3] FREE UNIV BRUSSELS,SCH MED,INST INTERDISCIPLINARY RES,BIOL UNIT,B-1050 BRUSSELS,BELGIUM

来源：

ARCHIVES OF DERMATOLOGICAL RESEARCH | 1992年 / 284卷 / 04期

关键词：

S-PHASE; CYCLIN IMMUNOSTAINING; H-3-THYMIDINE PULSE LABELING;

D O I：

10.1007/BF00375801

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Cyclin/PCNA (proliferating cell nuclear antigen) is a nuclear protein strongly associated with DNA replication sites. Under methanol fixation, cyclin immunostaining might serve as a specific marker for S-phase cells. Associated with single labelling with H-3-thymidine, this immunodetection represents an easy method for measuring S-phase duration. Its application to six samples of normal human epidermis allowed us to measure an S phase of 9.7 +/- 0.3 h, a result in fair agreement with a previous estimate of 10.2 h obtained from a classical double-labelling technique with two different concentrations of H-3-thymidine.

引用

页码：238 / 241

页数：4

共 20 条

[1] IDENTIFICATION OF A NUCLEAR AND OF A CYTOPLASMIC POLYPEPTIDE WHOSE RELATIVE PROPORTIONS ARE SENSITIVE TO CHANGES IN THE RATE OF CELL-PROLIFERATION [J].