GROWTH OF HUMAN X HUMAN HYBRIDOMAS IN PROTEIN-FREE MEDIUM SUPPLEMENTED WITH ETHANOLAMINE

被引:14
作者
COLE, SPC
VREEKEN, EH
MIRSKI, SEL
CAMPLING, BG
机构
[1] ONTARIO CANC TREATMENT & RES FDN, KINGSTON REG CANC CTR, KING ST W, KINGSTON K7L 2V7, ONTARIO, CANADA
[2] QUEENS UNIV, DEPT ONCOL, KINGSTON K7L 3N6, ONTARIO, CANADA
[3] QUEENS UNIV, DEPT MICROBIOL & IMMUNOL, KINGSTON K7L 3N6, ONTARIO, CANADA
[4] QUEENS UNIV, DEPT MED, KINGSTON K7L 3N6, ONTARIO, CANADA
关键词
D O I
10.1016/0022-1759(87)90101-3
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A protein-free medium was developed which supports the growth of human .times. human hybridomas derived from the fusion partner KR-4. THis medium consists of a 1:1 mixture of Iscove''s modified Dulbecco''s medium and Ham''s F-12 medium supplemented with 300 .mu.M ethanolamine. The addition of ethanolamine was essential to maintain cell viability and proliferation. Phosphoethanolamine could not substitute for ethanolamine. Although the maximal cell density achieved in protein-free medium was lower than that in medium supplemented with albumin and transferrin, immunoglobulin production was still supported. THis protein-free medium may be useful for large-scale cultivation and for metabolic and immunologic studies of human hybridomas.
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页码:29 / 35
页数:7
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