THE ADSORPTION OF BACTERIA TO IMMOBILIZED LECTINS

被引：22

作者：

PATCHETT, RA

KELLY, AF

KROLL, RG

机构：

[1] ARFC, Reading Laboratory, Institute of Food Research, Reading, Shinfield

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1991年 / 71卷 / 03期

关键词：

D O I：

10.1111/j.1365-2672.1991.tb04459.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The agglutination of a selection of bacteria by some lectins was examined. The lectin from Codium fragile agglutinated seven strains of Salmonella typhimurium. The lectin from Helix pomatia agglutinated eight of 12 strains of Listeria monocytogenes and a further two strains gave a weak agglutination reaction. Helix pomatia lectin conjugated to magnetic microspheres enabled the adsorption of L. monocytogenes from suspension with subsequent elution by the competing ligand N-acetyl galactosamine. Affinity chromatography of a suspension of L. monocytogenes through a column of H. pomatia lectin immobilized on agarose, also adsorbed cells and enabled subsequent elution with N-acetyl galactosamine. The column technique enabled the more rapid adsorption of bacteria perhaps because of improved interactions between bacteria and immobilized lectin.

引用

页码：277 / 284

页数：8

共 17 条

[1]

BIRD JA, 1989, SOC APPLIED BACTERIO, V25, P165

[2]

DANIELS SL, 1972, DEV IND MICROBIOL, V13, P211

[3] DIAGNOSTIC-VALUE OF INTERACTIONS BETWEEN MEMBERS OF THE FAMILY NEISSERIACEAE AND LECTINS [J].