ELECTRON IMMUNOCYTOCHEMICAL LOCALIZATION OF ACTINOBACILLUS-ACTINOMYCETEMCOMITANS LEUKOTOXIN

被引：46

作者：

BERTHOLD, P

FORTI, D

KIEBA, IR

ROSENBLOOM, J

TAICHMAN, NS

LALLY, ET

机构：

[1] Research Center for Oral Biology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania

来源：

ORAL MICROBIOLOGY AND IMMUNOLOGY | 1992年 / 7卷 / 01期

关键词：

ELECTRON MICROSCOPY; IMMUNOGOLD; LEUKOTOXIN; ORAL BACTERIA;

D O I：

10.1111/j.1399-302X.1992.tb00015.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

The Actinobacillus actinomycetemcomitans leukotoxin was localized in A. actinomycetemcomitans bacteria using an electron immunocytochemical thin-section labeling method. An immuno-dot blot procedure was initially used to ascertain the optimal specimen fixation. This consisted of a periodate-lysine-paraformaldehyde (2%) fixative in a phosphate buffer followed by embedding in LR White. Affinity-purified toxin was used to produce a monospecific polyclonal antibody. The reaction sites were visualized with a colloidal gold-tagged reporter antibody. The leukotoxin was found to be localized either in the cell envelope and/or in membranous vesicles on the outer surface of the bacterial cell. These results support previous observations indicating the same location.

引用

页码：24 / 27

页数：4

共 28 条

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