A SMALL CHROMOSOME OF LEISHMANIA (VIANNIA) BRAZILIENSIS CONTAINS MULTICOPY SEQUENCES WHICH ARE COMPLEX SPECIFIC

被引：9

作者：

ERESH, S ^{[1
]}

MENDOZALEON, A ^{[1
]}

BARKER, DC ^{[1
]}

机构：

[1] UNIV CAMBRIDGE, DEPT PATHOL,MOLTENO LABS,MRC,OUTSTAT NIMR, TENNIS COURT RD, CAMBRIDGE CB2 1QP, ENGLAND

来源：

ACTA TROPICA | 1993年 / 55卷 / 1-2期

基金：

英国医学研究理事会;

关键词：

LEISHMANIA (V) BRAZILIENSIS; KARYOTYPE; SMALL CHROMOSOME; REPETITIVE SEQUENCES;

D O I：

10.1016/0001-706X(93)90046-E

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Orthogonal Field Alternating Gel Electrophoresis (OFAGE) has been used to show a band of approximately 260 kb which is stained intensely with ethidium bromide in Leishmania (V.) braziliensis stock M2903. This small chromosome (sc-2903), as well as a 50 kb and a 200 kb chromosome seen in L. (L.) mexicana and L. (L.) amazonensis, respectively, are stably maintained and linear. When used as a hybridisation probe, sc-2903 showed homology to large chromosomal DNA bands and to a multiplicity of genomic fragments in all braziliensis stocks tested, indicating either different sequences, different copy numbers or both but no hybridisation to mexicana stocks. It is possible that these sequences are present in all members of the braziliensis complex and are not related to LD1 or any other previously published small chromosome sequences. However, at least one clone isolated from a sc-2903 library recognised genomic DNA of stocks belonging to the braziliensis, mexicana and donovani complexes. Our results suggest that the clone carries sequence(s) that are repeated and shared between stocks of different complexes but with a variable genomic distribution.

引用

页码：33 / 46

页数：14

共 44 条

[1]

Ashford R.W., 1987, P365

[2] DNA DIAGNOSIS OF HUMAN LEISHMANIASIS [J].