SOLVENT AND SOLID-PHASE EXTRACTION OF NATURAL AND SYNTHETIC CORTICOIDS IN HUMAN URINE

被引：31

作者：

SANTOSMONTES, A ^{[1
]}

GONZALOLUMBRERAS, R ^{[1
]}

GASCOLOPEZ, AI ^{[1
]}

IZQUIERDOHORNILLOS, R ^{[1
]}

机构：

[1] UNIV COMPLUTENSE MADRID,FAC QUIM,DEPT QUIM ANALIT,E-28040 MADRID,SPAIN

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 652卷 / 01期

关键词：

D O I：

10.1016/0378-4347(93)E0396-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Optimization of the main variables that affect solvent and solid-phase extraction processes, using disposable C18, cartridges and the non-ionic polymeric resin Serdolit AD-2, of human urine containing natural and synthetic corticoids is described. The data were obtained. from different HPLC separations of these compounds using calibration graphs obtained before and after extraction of these compounds. The procedures, including sample preconcentration, showed efficiencies over 90%. NaCl was used to avoid emulsion formation in solvent extraction. The results achieved using solvent and solid-phase extraction are discussed.

引用

页码：83 / 89

页数：7

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