DETERMINATION OF CEFTIOFUR AND ITS DESFUROYLCEFTIOFUR-RELATED METABOLITES IN SWINE TISSUES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：55

作者：

BECONIBARKER, MG ^{[1
]}

ROOF, RD ^{[1
]}

MILLERIOUX, L ^{[1
]}

KAUSCHE, FM ^{[1
]}

VIDMAR, TJ ^{[1
]}

SMITH, EB ^{[1
]}

CALLAHAN, JK ^{[1
]}

HUBBARD, VL ^{[1
]}

SMITH, GA ^{[1
]}

GILBERTSON, TJ ^{[1
]}

机构：

[1] CEPHAC,RES CTR,ST BENOIT,FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 673卷 / 02期

关键词：

D O I：

10.1016/0378-4347(95)00258-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An HPLC method was developed and validated for the determination of ceftiofur-related metabolites that have the potential to be microbiologically active in swine muscle, kidney, liver and fat. Its performance was evaluated against incurred-residue swine tissues. This method is based on the cleavage of the disulfide and/or thioester bonds between the metabolites and their conjugate sulfur containing moiety using dithioerythritol to yield desfuroylceftiofur, and further stabilization to desfuroylceftiofur acetamide. The limit of quantitation was 0.1 mu g ceftiofur equivalents/g tissue. The assay is specific for ceftiofur-related metabolites when evaluated against commercially available antibiotics for swine.

引用

页码：231 / 244

页数：14

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