DISTRIBUTION OF CALCITONIN GENE-RELATED PEPTIDE AND CALCITONIN-LIKE IMMUNOREACTIVITY IN TROUT

被引：45

作者：

FOUCHEREAUPERON, M ^{[1
]}

ARLOTBONNEMAINS, Y ^{[1
]}

TABOULET, J ^{[1
]}

MILHAUD, G ^{[1
]}

MOUKHTAR, MS ^{[1
]}

机构：

[1] CHU ST ANTOINE,CNRS,UA 163,PARIS,FRANCE

来源：

REGULATORY PEPTIDES | 1990年 / 27卷 / 02期

关键词：

Calcitonin gene-related peptide-like immunoreactivity; Calcitonin-like immunoreactivity; Fish; Molecular heterogeneity; Tissue distribution;

D O I：

10.1016/0167-0115(90)90037-W

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Radioimmunoassay and chromatography were used to study the occurrence of calcitonin gene-related peptide in various tissues of the rainbow trout, Salmo gairdnerii. The highest concentrations of the peptide were found in gill (1.68 ± 0.09 ng/mg protein) and in intestine (1.06 ± 0.4 ng/mg protein). Significant concentrations were also found in heart and stomach. The level in brain was very low. In trout, the plasma concentration accounted for 283 ± 82 pg/ml. Chromatographic analysis of the calcitonin gene-related peptide (CGRP)-like immunoreactivity occurring in gills showed that two molecular forms cross-reacted with the anti-human CGRP antibody, one co-eluting with the synthetic human CGRP. In addition, calcitonin in fish is not confined to the ultimobranchial organ but is also present in organs as heart, intestine, kidney, spleen and stomach. The evidence of CGRP in fish emphasizes the role of this hormone in evolution and leads us to investigate its physiological role in this species. © 1990.

引用

页码：171 / 179

页数：9

共 32 条

[1] ALTERNATIVE RNA PROCESSING IN CALCITONIN GENE-EXPRESSION GENERATES MESSENGER-RNAS ENCODING DIFFERENT POLYPEPTIDE PRODUCTS [J].