DIAGNOSTIC POTENTIAL FOR HUMAN MALIGNANCIES OF BACTERIALLY PRODUCED HTLV-I ENVELOPE PROTEIN

被引：43

作者：

SAMUEL, KP

LAUTENBERGER, JA

JORCYK, CL

JOSEPHS, S

WONGSTAAL, F

PAPAS, TS

机构：

[1] NCI, MOLEC ONCOL LAB, FREDERICK, MD 21701 USA

[2] NCI, TUMOR CELL BIOL LAB, BETHESDA, MD 20205 USA

来源：

SCIENCE | 1984年 / 226卷 / 4678期

关键词：

D O I：

10.1126/science.6208612

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Two regions of the gene for the human T-cell leukemia virus subgroup I (HTLV-I) envelope were expressed in Escherichia coli by use of the vector pJLA16. One corresponds to the carboxyl terminal region of the major envelope protein p46 and the other corresponds to the transmembrane protein p21E. Reactivity of the expressed protein with human serum was tested by the Western blot procedure. Each of 11 sera tested that were shown to contain antibodies to HTLV-I [human T-cell leukemia virus] or HTLV-II by an enzyme-linked immunosorbent assay recognized the bacterially synthesized envelope proteins. There was no reaction detected when 17 control sera were tested. This system will be useful for large-scale seroepidemiological surveys for HTLV-I and related human retroviruses.

引用

页码：1094 / 1097

页数：4

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