A COMPARISON OF PROCEDURES FOR ANALYZING MICROSATELLITE (CA)-REPEAT POLYMORPHISMS

被引：9

作者：

COHEN, BB

WALLACE, MR

CRICHTON, DN

机构：

[1] MRC Human Genetics Unit, Western General Hospital, Edinburgh, EH4 2XU, Crewe Road

来源：

MOLECULAR AND CELLULAR PROBES | 1992年 / 6卷 / 05期

关键词：

MICROSATELLITE; CA REPEAT; POLYMERASE CHAIN REACTION;

D O I：

10.1016/0890-8508(92)90039-Z

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Dinucleotide repeat sequences ('microsatellites') have been used as polymorphic genetic markers following amplification in the polymerase chain reaction (PCR). We have compared several methods of analysing the PCR products. The most reliable and unambiguous results were obtained when the PCR products were probed with a specific dinucleotide repeat olignucleotide, so that only the microsatellite-containing products were detectable. © 1992.

引用

页码：439 / 442

页数：4