ENDOTHELIAL NITRIC-OXIDE SYNTHASE IS MYRISTYLATED

被引：85

作者：

POLLOCK, JS

KLINGHOFER, V

FORSTERMANN, U

MURAD, F

机构：

[1] Vascular Biology Group, Abbott Laboratories, Abbott Park

来源：

FEBS LETTERS | 1992年 / 309卷 / 03期

关键词：

MYRISTIC ACID; PALMITIC ACID; EDRF; L-ARGININE; BOVINE AORTIC ENDOTHELIAL CELL;

D O I：

10.1016/0014-5793(92)80816-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The enzyme responsible for the synthesis of endothelium-derived relaxing factor and/or nitric oxide in the endothelium has been described as a particulate enzyme, whereas other isoforms of nitric oxide synthase are soluble enzymes. Here we are reporting that endothelial cells metabolically incorporate myristate (C14), but not palmitate (C16), into nitric oxide synthase. We are postulating that the endothelial-derived nitric oxide synthase is a particulate enzyme because of the fatty acid acylation of the protein which 'anchors' the enzyme into the membrane either directly or via another membrane-bound protein.

引用

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页码：402 / 404

页数：3

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