PREPARATIVE SEPARATION OF COMPONENTS OF THE COLOR ADDITIVE D-AND-C RED NO-28 (PHLOXINE B) BY PH-ZONE-REFINING COUNTERCURRENT CHROMATOGRAPHY

被引：30

作者：

WEISZ, A

ANDRZEJEWSKI, D

ITO, Y

机构：

[1] US FDA,OFF SCI ANAL & SUPPORT,WASHINGTON,DC 20204

[2] NHLBI,BIOPHYS CHEM LAB,BETHESDA,MD 20892

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 678卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0021-9673(94)87076-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A pH-zone-refining counter-current chromatographic method was developed for the preparative (multigram) separation and purification of components of the commercial color additive D&C Red No. 28 (phloxine B). The chromatography of 3 and 6 g of color additive yielded 1.07 and 4.06 g, respectively, of pure 2',4',5',7'-tetrabromo-4,5,6,7-tetrachlorofluorescein, the principal component of D&C Red No. 28. The importance of the quantity of retainer acid (trifluoroacetic acid) relative to the amount of salt in the color additive is discussed.

引用

页码：77 / 84

页数：8

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