INVITRO BIOSYNTHESIS OF STEROIDS, INCLUDING 11-DEOXYCORTISOL AND 5-ALPHA-PREGNANE-3-BETA, 7-BETA, 17,20-BETA-TETROL, BY OVARIES OF THE GOLDFISH CARASSIUS-AURATUS DURING THE STAGE OF OOCYTE FINAL MATURATION

被引:50
作者
KIME, DE [1 ]
SCOTT, AP [1 ]
CANARIO, AVM [1 ]
机构
[1] MAFF,DIRECTORATE FISHERIES RES,FISHERIES LAB,LOWESTOFT,SUFFOLK,ENGLAND
关键词
D O I
10.1016/0016-6480(92)90044-K
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
To examine the production of steroids with potential oocyte maturation-inducing or pheromonal activity in the goldfish (Carassius auratus) we have incubated mature ovaries of this species with 17-[3H]hydroxyprogesterone. The metabolites in the unconjugated, glucuronide, and sulfate fractions were identified by chromatography, microchemical reaction, and, in most cases, crystallization to constant specific activity. A major metabolite, present in all three fractions, was tentatively identified as 5α-pregnane-3β,7α,17,20β-tetrol. Although 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P) was found in only low yield (as a sulfate), the presence of the tetrol indicates that it is synthesized in high yield but very rapidly metabolized. The relative proportions of 17,20α-dihydroxy-4-pregnen-3-one (17,20α-P), 11-deoxycortisol (17,21-dihydroxy-4-pregnen-3,20-dione) and 17,20β,21-trihydroxy-4-pregnen-3-one (17,20β,21-P) varied significantly between incubations and may be affected by the maturational state of the ovary or the method used to stimulate oocyte maturation. Testosterone was present predominantly as its glucuronide. Significant production of glucuronides and sulfates was observed in all incubations. Twenty-five to 30% of the radioactivity remained associated with the tissue, but the distribution of activity between the metabolites did not differ greatly from that found in the medium. These results indicate that 11-deoxycorisol and its 20β-reduced derivative (17,20β,21-P) may be significant in spawning female goldfish. © 1992.
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页码:375 / 384
页数:10
相关论文
共 25 条
[1]   SYNTHESIS OF 20-ALPHA-HYDROXYLATED STEROIDS BY OVARIES OF THE DAB (LIMANDA-LIMANDA) [J].
CANARIO, AVM ;
SCOTT, AP .
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1989, 76 (01) :147-158
[2]   IDENTIFICATION OF, AND DEVELOPMENT OF RADIOIMMUNOASSAYS FOR 17-ALPHA,21-DIHYDROXY-4-PREGNENE-3,20-DIONE AND 3-ALPHA,17-ALPHA,21-TRIHYDROXY-5-BETA-PREGNAN-20-ONE IN THE OVARIES OF MATURE PLAICE (PLEURONECTES-PLATESSA) [J].
CANARIO, AVM ;
SCOTT, AP .
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1990, 78 (02) :273-285
[3]  
GOETZ FW, 1983, FISH PHYSIOL, V9, P117
[4]   THE MODULATORY EFFECT INVITRO OF ESTRADIOL-17-BETA, TESTOSTERONE OR CORTISOL ON THE OUTPUT OF 17-ALPHA-HYDROXY-20-BETA-DIHYDROPROGESTERONE BY RAINBOW-TROUT (SALMO-GAIRDNERI) OVARIAN FOLLICLES STIMULATED BY THE MATURATIONAL GONADOTROPIN S-GTH [J].
JALABERT, B ;
FOSTIER, A .
REPRODUCTION NUTRITION DEVELOPMENT, 1984, 24 (02) :127-136
[5]   INVITRO OOCYTE MATURATION AND OVULATION IN RAINBOW-TROUT (SALMO-GAIRDNERI), NORTHERN PIKE (ESOX-LUCIUS), AND GOLDFISH (CARASSIUS-AURATUS) [J].
JALABERT, B .
JOURNAL OF THE FISHERIES RESEARCH BOARD OF CANADA, 1976, 33 (04) :974-988
[6]  
KAGAWA H, 1983, B JPN SOC SCI FISH, V49, P1783
[8]   5-ALPHA-PREGNANE-3-BETA,7-ALPHA,17,20-ALPHA-TETROLS AND 17-20-BETA-TETROLS AS METABOLITES OF PROGESTERONE AND 17-HYDROXYPROGESTERONE IN CARP (CYPRINUS-CARPIO) OVARIAN INCUBATIONS [J].
KIME, DE ;
VENKATESH, B ;
TAN, CH .
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1991, 84 (03) :401-404
[9]  
KIME DE, 1991, 4TH P INT S REPR PHY, P77
[10]   HORMONE CHANGES DURING OVULATION AND EFFECTS OF STEROID-HORMONES ON PLASMA GONADOTROPIN-LEVELS AND OVULATION IN GOLDFISH [J].
KOBAYASHI, M ;
AIDA, K ;
HANYU, I .
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1987, 67 (01) :24-32