DIVERGENT EFFECTS OF PHORBOL ESTERS AND INSULIN ON INSULIN-LIKE GROWTH-FACTOR BINDING PROTEIN-1 (IGFBP-1) PRODUCTION AND MESSENGER-RNA IN RAT H4IIE HEPATOMA-CELLS

被引：16

作者：

UNTERMAN, TG

LACSON, RG

JENTEL, JJ

OEHLER, DT

机构：

[1] VET ADM W SIDE MED CTR,CHICAGO,IL 60612

[2] UNIV ILLINOIS,COLL MED,MED RES SERV,CHICAGO,IL 60612

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 182卷 / 01期

关键词：

D O I：

10.1016/S0006-291X(05)80139-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

125I-IGF-I binding assay, western ligand and immunoblotting, and northern analysis of total RNA reveal that phorbol ester agonists of protein kinase C rapidly enhance IGFBP-1 production and increase the abundance of IGFBP-1 mRNA in rat H4IIE hepatoma cells. In combination with insulin, a potent inhibitor of IGFBP-1 gene transcription, this early effect of phorbol esters is dominant. These results demonstrate divergent regulation of IGFBP-1 by phorbol esters and insulin and indicate that protein kinase C may play a critical role in the regulation of IGFBP-1 and modulation IGF bioactivity in metabolic disease. © 1992 Academic Press, Inc.

引用

页码：262 / 268

页数：7

共 27 条

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