INHIBITION OF RNA POLYMERASE-II TRANSCRIPTION BY OLIGONUCLEOTIDE-RECA PROTEIN FILAMENTS TARGETED TO PROMOTER SEQUENCES

被引：4

作者：

GOLUB, EI ^{[1
]}

RADDING, CM ^{[1
]}

WARD, DC ^{[1
]}

机构：

[1] YALE UNIV,SCH MED,DEPT MOLEC BIOPHYS & BIOCHEM,NEW HAVEN,CT 06510

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 15期

关键词：

HUMAN IMMUNODEFICIENCY VIRUS LONG TERMINAL REPEAT; PARVOVIRUS; MINUTE VIRUS OF MICE;

D O I：

10.1073/pnas.90.15.7186

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In the presence of RecA protein, which plays a major role in genetic recombination in Escherichia coli, an oligodeoxyribonucleotide can find its homologous counterpart in double-stranded DNA and form triple-stranded structures. A triple-stranded structure formed by an oligonucleotide with a sequence overlapping essential regulatory elements of a viral promoter, such as TATA or GC boxes, inhibited in vitro transcription driven by RNA polymerase II. An oligonucleotide with eight nucleotides homologous to its target suppressed RNA polymerase II activity in HeLa cell extracts. This procedure offers a potential alternative to the usual mutational analysis of transcriptional promoters.

引用

页码：7186 / 7190

页数：5

共 25 条

[1] TRANSCRIPTIONAL ANALYSIS OF MINUTE VIRUS OF MICE P4 PROMOTER MUTANTS [J].

AHN, JK ;

GAVIN, BJ ;

KUMAR, G ;

WARD, DC .

JOURNAL OF VIROLOGY, 1989, 63 (12) :5425-5439

[2] 2ND STRUCTURAL MOTIF FOR RECOGNITION OF DNA BY OLIGONUCLEOTIDE-DIRECTED TRIPLE-HELIX FORMATION [J].

BEAL, PA ;

DERVAN, PB .

SCIENCE, 1991, 251 (4999) :1360-1363

[3] A GC-BOX MOTIF UPSTREAM OF THE B19 PARVOVIRUS UNIQUE PROMOTER IS IMPORTANT FOR INVITRO TRANSCRIPTION [J].

BLUNDELL, MC ;

ASTELL, CR .

JOURNAL OF VIROLOGY, 1989, 63 (11) :4814-4823

[4] 5 INTERMEDIATE COMPLEXES IN TRANSCRIPTION INITIATION BY RNA POLYMERASE-II [J].

BURATOWSKI, S ;

HAHN, S ;

GUARENTE, L ;

SHARP, PA .

CELL, 1989, 56 (04) :549-561

[5] SITE-SPECIFIC OLIGONUCLEOTIDE BINDING REPRESSES TRANSCRIPTION OF THE HUMAN C-MYC GENE INVITRO [J].

COONEY, M ;

CZERNUSZEWICZ, G ;

POSTEL, EH ;

FLINT, SJ ;

HOGAN, ME .

SCIENCE, 1988, 241 (4864) :456-459

[6] SELECTIVE CLEAVAGE OF HUMAN DNA - RECA-ASSISTED RESTRICTION ENDONUCLEASE (RARE) CLEAVAGE [J].

FERRIN, LJ ;

CAMERINIOTERO, RD .

SCIENCE, 1991, 254 (5037) :1494-1497

[7] DIFFERENCES IN THE BASAL ACTIVITY OF THE LONG TERMINAL REPEAT DETERMINE DIFFERENT REPLICATIVE CAPACITIES OF 2 CLOSELY RELATED HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ISOLATES [J].

GOLUB, EI ;

LI, GR ;

VOLSKY, DJ .

JOURNAL OF VIROLOGY, 1990, 64 (08) :3654-3660

[8] JOINTS FORMED BY RECA PROTEIN FROM OLIGONUCLEOTIDES AND DUPLEX DNA BLOCK INITIATION AND ELONGATION OF TRANSCRIPTION [J].

GOLUB, EI ;

WARD, DC ;

RADDING, CM .

NUCLEIC ACIDS RESEARCH, 1992, 20 (12) :3121-3125

[9]

HOZIKOSKI M, 1992, P NATL ACAD SCI USA, V89, P1060

[10] THE SYNAPSIS EVENT IN THE HOMOLOGOUS PAIRING OF DNAS - RECA RECOGNIZES AND PAIRS LESS THAN ONE HELICAL REPEAT OF DNA [J].

HSIEH, P ;

CAMERINIOTERO, CS ;

CAMERINIOTERO, RD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (14) :6492-6496

← 1 2 3 →