MEASUREMENT OF RED BLOOD CELL-VITAMIN-B12 - A STUDY OF THE CORRELATION BETWEEN INTRACELLULAR B12 CONTENT AND CONCENTRATIONS OF PLASMA HOLOTRANSCOBALAMIN-II

被引：19

作者：

TISMAN, G ^{[1
]}

VU, T ^{[1
]}

AMIN, J ^{[1
]}

LUSZKO, G ^{[1
]}

BRENNER, M ^{[1
]}

RAMOS, M ^{[1
]}

FLENER, V ^{[1
]}

CORDTS, V ^{[1
]}

BATEMAN, R ^{[1
]}

MALKIN, S ^{[1
]}

BROWDER, T ^{[1
]}

机构：

[1] ADV TREATMENT CANC & BLOOD DIS CTR,WHITTIER,CA

来源：

AMERICAN JOURNAL OF HEMATOLOGY | 1993年 / 43卷 / 03期

关键词：

COBALAMIN; NEGATIVE B12 BALANCE; COBALAMIN DEFICIENCY;

D O I：

10.1002/ajh.2830430313

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We have recently reported a new and rapid assay to measure plasma holotranscobalamin II (holoTC II) as a means of exploring vitamin B12 status. In order to further evaluate the significance of plasma holoTC II in determining tissue cobalamin, we have chosen the red blood cell-vitamin B12 (RBC-B12) assay as a measure of tissue vitamin B12 content and studied the relationship between RBC-B12 and plasma holoTC II levels. Plasma holoTC II and RBC-B12 concentrations were concomitantly assayed in 20 hematologically normal controls and cancer patients. In our groups of controls, the mean value of RBC-B12 was determined as 241 +/- 51 pg/ml of packed erythrocytes (PE) with a range varying from 180 to 355 pg/ml PE. Preliminary results obtained in 32 cancer patients revealed lower holoTC II and RBC-B12 levels than the control group and a required threshold value of 70 pg/ml of holoTC II in order to maintain a normal RBC-B12 greater than 180 pg/ml PE.

引用

页码：226 / 229

页数：4

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