BINDING OF BACILLUS-SPHAERICUS BINARY TOXIN TO A SPECIFIC RECEPTOR ON MIDGUT BRUSH-BORDER MEMBRANES FROM MOSQUITO LARVAE

被引:112
作者
NIELSENLEROUX, C [1 ]
CHARLES, JF [1 ]
机构
[1] INST PASTEUR, 25 RUE DR ROUX, F-75724 PARIS 15, FRANCE
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1992年 / 210卷 / 02期
关键词
D O I
10.1111/j.1432-1033.1992.tb17458.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The presence of specific receptors for Bacillus sphaericus binary toxin on brush-border membrane fractions (BBMF) from Culex pipiens larvae midgut cells was demonstrated by an in vitro binding assay. Both activated and radiolabelled polypeptides from the 51-kDa and 42-kDa binary toxin of B. sphaericus 1593 specifically bound to BBMF. Direct binding and homologous competition experiments indicated a single class of B. sphaericus toxin receptors, with a dissociation constant (K(d)) of approximately 20 nM and a maximum binding capacity (B(max)) of approximately 7 pmol/mg BBMF protein. The sugars GalNAc, GlcNAc and N-acetyl neuraminic acid had no detectable inhibitory effect on toxin binding to C. pipiens BBMF. Binding experiments with the non-susceptible mosquito species Aedes aegypti failed to detect significant binding of B. sphaericus binary toxin to A. aegypti BBMF.
引用
收藏
页码:585 / 590
页数:6
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