ONE-STEP SANDWICH ENZYME-IMMUNOASSAY FOR SOLUBLE HUMAN THROMBOMODULIN USING MONOCLONAL-ANTIBODIES

被引：63

作者：

KODAMA, S ^{[1
]}

UCHIJIMA, E ^{[1
]}

NAGAI, M ^{[1
]}

MIKAWATANI, K ^{[1
]}

HAYASHI, T ^{[1
]}

SUZUKI, K ^{[1
]}

机构：

[1] UNIV TOKUSHIMA, INST ENZYME RES, TOKUSHIMA 770, JAPAN

来源：

CLINICA CHIMICA ACTA | 1990年 / 192卷 / 03期

关键词：

Monoclonal antibodies; Sandwich enzyme immunoassay; Soluble thrombomodulin; Systemic lupus erythematosus;

D O I：

10.1016/0009-8981(90)90221-D

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Six monoclonal antibodies for human thrombomodulin (TM) were prepared. All of them recognized an elastase-digested fragment of TM which contains 6 epidermal growth factor (EGF)-like structural domains. We developed a one-step sandwich enzyme immunoassay for soluble TM by using 2 antibodies; one of them, which inhibited thrombin-binding to TM, was fixed to polystyrene balls, and the other, which did not inhibit the thrombin-binding, but inhibited the protein C-activating cofactor activity of TM, was used as peroxidase-labeled conjugate. The sensitivity of this assay was 1 μg/l for soluble TM. The level of soluble TM was found to be significantly increased in sera of patients with systemic lupus erythematosus in comparison to the level in sera of healthy subjects. © 1990.

引用

页码：191 / 200

页数：10

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