ANALYSIS OF A CR-1 (CHICKEN REPEAT) SEQUENCE FLANKING THE 5' END OF THE GENE ENCODING ALPHA-SKELETAL ACTIN

Genomic Southern blots of chicken DNA, probed with radiolabeled DNA fragments that flank the 5′ end of the asa gene encoding chicken α-skeletal actin, indicate the presence of repetitive nucleotide sequences. Sequence analysis of this region reveals a member of the CR1 family of middle-repetitive elements. A 186-bp restriction fragment carrying the 3′-end of this CR1 element binds factor(s) present in nuclear extracts, as assayed band-shift electrophoresis. However, the CR1 repeat has limited influence on transcription from the α-skeletal actin promoter, as assessed by CAT assays of transfected chicken myoblasts and fibroblasts. © 1990.