FIBROBLAST GROWTH-FACTOR (FGF)-2 SENSE AND ANTISENSE MESSENGER-RNA AND FGF RECEPTOR-TYPE-1 MESSENGER-RNA ARE PRESENT IN THE EMBRYONIC AND ADULT-RAT NERVOUS-SYSTEM - SPECIFIC DETECTION BY NUCLEASE PROTECTION ASSAY

被引：24

作者：

GROTHE, C

MEISINGER, C

机构：

[1] Institute of Anatomy, University of Freiburg, D-79104 Freiburg

来源：

NEUROSCIENCE LETTERS | 1995年 / 197卷 / 03期

关键词：

FIBROBLAST GROWTH FACTOR; RECEPTOR; MESSENGER-RNA; ANTISENSE; RAT; DEVELOPMENT; NUCLEASE PROTECTION ASSAY;

D O I：

10.1016/0304-3940(95)11917-L

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

In the present study, we attempted to clarify the controversial question whether basic fibroblast growth factor (bFGF FGF-2) mRNA is present or absent in the embryonic central nervous system (CNS). For this purpose we analyzed the expression of the FGF-2 mRNA in the embryonic and adult forebrain, brainstem, and spinal cord using the highly specific ribonuclease protection assay. Using this method we were able to detect FGF-2 mRNA in the rat CNS of embryonic day (E) 16 and 17, however, at lower levels compared to adult FGF-2 mRNA levels. In addition, we show that a FGF-2 antisense transcript is expressed in embryonic CNS tissue. Furthermore, using this method, we demonstrate FGF receptor 1 mRNA in the rat embryonic and adult CNS. The presence of FGF-2 and FGF receptor 1 suggests a physiological role for this growth factor during the development of the embryonic CNS.

引用

页码：175 / 178

页数：4

共 29 条

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