INVITRO INDUCTION AND EXPRESSION OF INTERLEUKIN-2 RECEPTOR IN A CLONAL T-HELPER CELL-DIFFERENTIATION MODEL

被引：32

作者：

BISMUTH, G ^{[1
]}

LECLERCQ, L ^{[1
]}

DUPHOT, M ^{[1
]}

MOREAU, JL ^{[1
]}

THEZE, J ^{[1
]}

机构：

[1] INST PASTEUR, DEPT IMMUNOL, UNITE IMMUNOGENET CELLULAIRE, 25 RUE DOCTEUR ROUX, F-75724 PARIS 15, FRANCE

来源：

CELLULAR IMMUNOLOGY | 1984年 / 86卷 / 01期

关键词：

D O I：

10.1016/0008-8749(84)90362-9

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Induction and expression of interleukin 2 (IL 2) receptor were studied using a poly(Glu60Ala30Tyr10) (GAT)-specific T cell clone of mouse origin. This clone (52-3) was characterized and it exhibits functional properties of T helper (TH) cells; it leads to a specific anti-DNP [anti-dinitrophenyl] response in the presence of DNP-GAT and DNP-primed B cells, and it secretes biological activities which can induce polyclonal B cell proliferation and IgM secretion. In vitro this clone mimics the activation stages of normal T lymphocytes and can be obtained under 2 states of differentiation, depending on the antigen-specific signal provided by antigen-presenting cells (APC). The expression of IL 2 receptor by this clone was studied by its response to IL 2, its capacity to absorb IL 2 bioactivity and its reactivity with monoclonal antibody 7D4 specific for mouse IL 2 receptor. The unstimulated state does not express the IL 2 receptor, while the activated state does. Clone 52-3 was compared with clone 14-1.6 that derives from a TH cell line and expresses the IL 2 receptor constitutively. 52-3 offers a good experimental model for studying in vitro, in a clonal TH cell population, the detailed mechanism of IL 2 receptor induction.

引用

页码：90 / 100

页数：11

共 21 条

[1] T-CELL-DEPENDENT B-CELL STIMULATION IS H-2 RESTRICTED AND ANTIGEN DEPENDENT ONLY AT THE RESTING B-CELL LEVEL [J].