VOLTAGE-DEPENDENT PUMP CURRENTS OF THE SARCOPLASMIC-RETICULUM CA-2+-ATPASE IN PLANAR LIPID-MEMBRANES

Sarcoplasmic reticulum vesicles containing largely Ca2+-ATPase were incorporated into planar lipid membranes. The ATPase was activated by a UV flash-induced concentration jump of ATP from a photolabile caged ATP. Under these conditions stationary pump currents were observed. The dependence of these pump currents on applied voltages was investigated. The current-voltage curve of the Ca2+-ATPase shows monotonously increasing pump currents with increasing positive potentials of the ATP containing compartment. This indicates the existence of electrogenic steps in the direction of the transported Ca2+ ions. From the extrapolated reversal potentials of the curve is concluded that less than four positive net charges are transported per hydrolyzed ATP. © 1990.