ULTRASTRUCTURAL IDENTIFICATION OF HUMAN MAST-CELLS RESEMBLING SKIN MAST-CELLS STIMULATED TO DEVELOP IN LONG-TERM HUMAN CORD BLOOD MONONUCLEAR-CELLS CULTURED WITH 3T3 MURINE SKIN FIBROBLASTS

被引：26

作者：

DVORAK, AM

FURITSU, T

KISSELLRAINVILLE, S

ISHIZAKA, T

机构：

[1] HARVARD UNIV,BETH ISRAEL HOSP,DEPT PATHOL,BOSTON,MA 02215

[2] HARVARD UNIV,SCH MED,DEPT PATHOL,BOSTON,MA 02115

[3] BETH ISRAEL HOSP,CHARLES A DANA RES INST,BOSTON,MA 02215

来源：

JOURNAL OF LEUKOCYTE BIOLOGY | 1992年 / 51卷 / 06期

关键词：

BASOPHIL; SECRETORY GRANULES; MACROPHAGE; INTERLEUKIN-3; EOSINOPHIL; ENDOTHELIAL CELL;

D O I：

10.1002/jlb.51.6.557

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Human mast cells developed in vitro when cord blood mononuclear cells were cocultured for 3 months with 3T3 embryonic mouse skin fibroblasts. The metachromatic cells that arose in these cultures contained histamine, a functional Fc(epsilon) receptor and granule proteases (tryptase, chymase), and they were definitively identified by the ultrastructural demonstration of crystal granules. We present a detailed ultrastructural analysis of this newly available system for the reliable development of human mast cells in vitro and provide criteria for definitive identification of the mast cell and basophil lineages in humans.

引用

页码：557 / 569

页数：13

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