POSTMORTEM DELAY EFFECTS ON NEUROGLIAL CELLS AND BRAIN MACROPHAGES FROM LEWIS RATS WITH ACUTE EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS - AN IMMUNOHISTOCHEMICAL AND CYTOCHEMICAL STUDY

被引：18

作者：

DEGROOT, CJA ^{[1
]}

THEEUWES, JWM ^{[1
]}

DIJKSTRA, CD ^{[1
]}

VANDERVALK, P ^{[1
]}

机构：

[1] GRAD SCH NEUROSCI, DEPT CELL BIOL & IMMUNOL, 1081 BT AMSTERDAM, NETHERLANDS

来源：

JOURNAL OF NEUROIMMUNOLOGY | 1995年 / 59卷 / 1-2期

关键词：

POSTMORTEM DELAY; EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS; MULTIPLE SCLEROSIS; BRAIN MACROPHAGE; ASTROCYTE; MICROGLIA;

D O I：

10.1016/0165-5728(95)00034-Y

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The effects of increasing postmortem delay (PMD) times on morphological, immunological and functional characteristics of various brain cells both in situ and in vitro were studied in postmortem brain tissue derived from rats with acute experimental allergic encephalomyelitis (EAE). A decline of the brain tissue structure was first noted after a PMD of 6 h. Radial glia in the cerebellum were frequently interrupted and retractions artifacts appeared around brain cells. However, even after the longest PMD interval of 18 h the quality of the cell and tissue structure was still good enough for immunohistochemical characterization. Immunohistochemical staining of frozen and fixed rat brain tissue sections resulted in an enhancement of the immunoreactivity after a PMD of 4 h, using a panel of mono and polyclonal antibodies directed against glial fibrillary acidic protein (GFAP), basement membranes (laminin), brain macrophage antigens (ED1 and ED2), and various immunologically important surface molecules, such as major histocompatibility complex (MHC) class II (Ia) antigen (OX6), CR3 complement receptor (ED8), and leukocyte common antigen (OX1). No increase in staining intensities with the ED1, ED8 and OX6 mAbs specific for macrophage antigens could be detected on brain macrophages that were isolated from brain tissue of rats with EAE obtained after various PMD intervals. Irrespective of the PMD interval, viable astrocyte cell cultures were obtained with comparable staining intensities for GFAP. These cultured astrocytes were capable of ingesting Latex beads and were highly proliferative as measured by BrdU uptake, at all investigated PMDs. Thus, even after long PMD intervals, brain material can be used successfully. Other data suggest that the situation is similar to human brain material, even though the PMD times may be somewhat different.

引用

页码：123 / 134

页数：12

共 27 条

[1] Damoiseaux, Dopp, Neefjes, Beelen, Dijkstra, Heterogeneity of macrophages in the rat evidenced by variability in determinants: two new anti-rat macrophage antibodies against a heterodimer of 160 and 95 kD (CD11/CD18), J. Leucocyte Biol., 46, pp. 556-564, (1989)
[2] Damoiseaux, Barbe, Dopp, Dijkstra, Characterization and expression of the antigen present on resident rat macrophages recognized by monoclonal antibody ED2, Immunobiol., 182, pp. 88-99, (1990)
[3] Damoiseaux, Dopp, Calame, Chao, MacPherson, Dijkstra, Rat macrophage lysosomal membrane antigen recognized by monoclonal antibody ED1, Immunol., 83, pp. 140-147, (1994)
[4] De Groot, Sminia, Dijkstra, Isolation and characterization of brain macrophages from the central nervous system of newborn and adult rats and of rats with experimental allergic encephalomyelitis, Immunobiology, 179, pp. 314-327, (1989)
[5] De Groot, Van der Pal, Lopes-Cardozo, Sminia, Dijkstra, Interferon-γ induced antigen expression on cultured neuroglial cells and brain macrophages from rat spinal cord and cerebrum, International Journal of Neuroscience, 56, pp. 53-65, (1991)
[6] Dijkstra, Dopp, Joling, Kraal, The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2, and ED3, Immunology, 54, pp. 589-599, (1985)
[7] Eng, The glial fibrillary acidic protein the major protein constitute of glial filaments, Scandinavian Journal of Immunology, 15, pp. 41-51, (1982)
[8] Eng, Ghirnikar, GFAP and astrogliosis, Brain Pathol., 4, pp. 229-237, (1994)
[9] Foidart, Bere, Yaar, Distribution and immunoelectron microscopic localisation of laminin, a non-collagenous basement membrane glycoprotein, Lab. Invest., 42, pp. 336-342, (1980)
[10] Graeber, Streit, Kreutzberg, Identity of ED2-positive perivascular cells in rat brain, J. Neurosci. Res., 22, pp. 103-106, (1989)

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