PROGRESSIVE B-CELL CHRONIC LYMPHOCYTIC-LEUKEMIA FREQUENTLY EXHIBITS ABERRANT P53 EXPRESSION

被引:14
作者
AGUILARSANTELISES, M [1 ]
MAGNUSSON, KP [1 ]
WIMAN, KG [1 ]
MELLSTEDT, H [1 ]
JONDAL, M [1 ]
机构
[1] KAROLINSKA HOSP, DEPT ONCOL, S-10401 STOCKHOLM, SWEDEN
关键词
D O I
10.1002/ijc.2910580403
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
We have analysed the p53 status in non-progressive and progressive chronic B-cell leukaemia (B-CLL) by ELISA, immunoprecipitation, FACS and cDNA sequencing in relation to in vitro proliferation in response to Staphylococcus aureus strain Cowan I (SAC) and IL-2. In FACS, cells from progressive leukaemia were found to over-express p53 with a conformation recognized by PAb240. In a PAb240-based ELISA, 60% of progressive B-CLL were positive. DNA sequencing of p53 exons 5 to 9 revealed a codon 179 His to Gin change in one of the ELISA-positive, progressive B-CLL but failed to reveal any mutations in 4 other ELISA-positive, progressive B CLL. Among progressive B-CLL populations, 10/14 responded by proliferation in vitro to SAC/IL-2. In non-progressive cells, low levels of p53 were found by FACS, none was positive in the PAb240 ELISA and only one case showed a weak proliferative response to SAC/IL-2. Low p53 expression was also seen in different types of normal B cells, resting and activated, and in EBV-transformed B-cell lines, in contrast to the high expression observed in Burkitt lymphoma cell lines with verified p53 mutations. We conclude that progressive B-CLL is characterized by aberrant P53 expression which may be a significant prognostic factor. (C) 1994 Wiley-Liss, Inc.
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页码:474 / 479
页数:6
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