PHOTOCHEMICAL LINKAGE OF ANTIBODIES TO SILICON CHIPS

被引：28

作者：

SUNDARABABU, G ^{[1
]}

GAO, H ^{[1
]}

SIGRIST, H ^{[1
]}

机构：

[1] UNIV BERN,INST BIOCHEM,CH-3012 BERN,SWITZERLAND

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1995年 / 61卷 / 06期

关键词：

D O I：

10.1111/j.1751-1097.1995.tb09867.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Antibodies and antigen binding fragments thereof were photochemically immobilized on surface-modified silicon chips of 5 x 5 mm size. Silicon surface-grafted diazirines and benzophenones formed covalent bonds with the immunoreagents on light activation. Photolithographic immobilization of monoclonal antibodies in aqueous media was achieved on silicon chips by activating surface-grafted benzophenones. The presence of bovine serum albumin during irradiation reduced nonspecific adsorption of the immunoreagents and retained the immunoactivity of the photoimmobilized molecules.

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页码：540 / 544

页数：5

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