ACCUMULATION OF SULFOGLYCOLIPIDS IN HYPEROSMOSIS-RESISTANT CLONES DERIVED FROM THE RENAL EPITHELIAL-CELL LINE MDCK (MADIN-DARBY CANINE KIDNEY-CELL)

被引：18

作者：

NIIMURA, Y ^{[1
]}

ISHIZUKA, I ^{[1
]}

机构：

[1] TEIKYO UNIV,SCH MED,DEPT BIOCHEM,2-11-1 KAGA,ITABASHI KU,TOKYO 173,JAPAN

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1991年 / 100卷 / 03期

关键词：

D O I：

10.1016/0305-0491(91)90216-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. Two clones (osmR-A and osmR-B) resistant to hyperosmotic media of 700 and 800 mosmol/l, respectively, were selected from Madin-Darby canine kidney (MDCK) cells. 2. When cultured in isosmotic medium (300 mosmol/l), the concentration of galactosyl sulfatide and lactosyl sulfatide in these hyperosmosis-resistant clones was 3.4-5.9 times higher than in the wild-type MDCK. The rate of incorporation of [S-35]sulfate into sulfolipids of osmR-A and osmR-B was 1.9-6.7 times higher than MDCK. 3. The stimulation of incorporation into sulfolipids by hyperosmotic culture was completely inhibited by cycloheximide. The pulse-chase studies indicated decreased turnover rate of sulfolipids in osmR-A.

引用

页码：535 / 541

页数：7

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