THE CD8-ALPHA LOCUS IS LOCATED ON THE TELOMERE SIDE OF THE IMMUNOGLOBULIN-KAPPA LOCUS AT A DISTANCE OF 2 MB

被引：15

作者：

WEICHHOLD, GM

HUBER, C

PARNES, JR

ZACHAU, HG

机构：

[1] STANFORD UNIV,MED CTR,SCH MED,DIV IMMUNOL,STANFORD,CA 94305

[2] UNIV MUNICH,INST PHYSIOL CHEM,W-8000 MUNICH 2,GERMANY

来源：

GENOMICS | 1993年 / 16卷 / 02期

关键词：

D O I：

10.1006/geno.1993.1218

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The immunoglobulin κ and CD8α loci had been mapped by in situ hybridization to the short arm of chromosome 2 at 2cen-p11.2 and 2p1, respectively, but probes derived from the two loci did not hybridize to the same large PFGE fragments. We have now succeeded in linking the two loci on a 3.0-Mb fragment from a partial NruI digest. A new hybridization probe located between the two loci provided additional linking fragments and allowed the distance to be determined as 2.0-2.2 Mb. © 1993 Academic Press. All rights reserved.

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页码：512 / 514

页数：3

相关论文

共 15 条

[1]

HUBER C, 1993, THESIS U MUNCHEN

[2] A HUMAN HOMOLOG OF THE MOUSE CD8 MOLECULE, LYT-3 - GENOMIC SEQUENCE AND EXPRESSION [J].

JOHNSON, P .

IMMUNOGENETICS, 1987, 26 (03) :174-177

[3] N-SEGMENT INSERTION AND REGION-DIRECTED SOMATIC HYPERMUTATION IN A KAPPA-GENE OF A T(2-8) CHROMOSOMAL TRANSLOCATION [J].

KLOBECK, HG ;

COMBRIATO, G ;

ZACHAU, HG .

NUCLEIC ACIDS RESEARCH, 1987, 15 (12) :4877-4888

[4] MAPPING IMMUNOGLOBULIN GENE-RELATED DNA PROBES TO THE CENTRAL REGION OF NORMAL AND PERICENTRICALLY INVERTED HUMAN CHROMOSOME-2 [J].

LAUTNERRIESKE, A ;

HAMEISTER, H ;

BARBI, G ;

ZACHAU, HG .

GENOMICS, 1993, 16 (02) :497-502

[5] THE ISOLATION AND SEQUENCE OF THE GENE ENCODING T8 - A MOLECULE DEFINING FUNCTIONAL CLASSES OF LYMPHOCYTES-T [J].

LITTMAN, DR ;

THOMAS, Y ;

MADDON, PJ ;

CHESS, L ;

AXEL, R .

CELL, 1985, 40 (02) :237-246

[6] PHYSICAL MAP OF THE HUMAN IMMUNOGLOBULIN-K LOCUS AND ITS IMPLICATIONS FOR THE MECHANISMS OF VK-JK REARRANGEMENT [J].

LORENZ, W ;

STRAUBINGER, B ;

ZACHAU, HG .

NUCLEIC ACIDS RESEARCH, 1987, 15 (23) :9667-9676

[7] LOCALIZATION OF HUMAN-IMMUNOGLOBULIN KAPPA-LIGHT CHAIN VARIABLE REGION GENES TO THE SHORT ARM OF CHROMOSOME-2 BY INSITU HYBRIDIZATION [J].

MALCOLM, S ;

BARTON, P ;

MURPHY, C ;

FERGUSONSMITH, MA ;

BENTLEY, DL ;

RABBITTS, TH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (16) :4957-4961

[8] STRUCTURE AND EXPRESSION OF THE GENE ENCODING CD8-ALPHA CHAIN (LEU-2/T8) [J].

NAKAYAMA, K ;

TOKITO, S ;

OKUMURA, K ;

NAKAUCHI, H .

IMMUNOGENETICS, 1989, 30 (05) :393-397

[9] POLYMORPHISMS AND HAPLOTYPES IN THE HUMAN IMMUNOGLOBULIN-KAPPA LOCUS [J].

PARGENT, W ;

SCHABLE, KF ;

ZACHAU, HG .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1991, 21 (08) :1829-1835

[10] A 2ND CHAIN OF HUMAN CD8 IS EXPRESSED ON PERIPHERAL-BLOOD LYMPHOCYTES [J].

SHIUE, L ;

GORMAN, SD ;

PARNES, JR .

JOURNAL OF EXPERIMENTAL MEDICINE, 1988, 168 (06) :1993-2005