DETERMINATION OF TANNIC-ACID AND ITS PHENOLIC METABOLITES IN BIOLOGICAL-FLUIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：45

作者：

ZHU, J

NG, J

FILIPPICH, LJ

机构：

[1] UNIV QUEENSLAND,SCH VET SCI,DEPT COMPAN ANIM MED & SURG,BRISBANE,QLD 4072,AUSTRALIA

[2] UNIV QUEENSLAND,SCH VET SCI,DEPT VET PATHOL,TOXICOL LAB,BRISBANE,QLD 4072,AUSTRALIA

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 577卷 / 01期

关键词：

D O I：

10.1016/0378-4347(92)80600-U

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for the identification and determination of tannic acid and its phenolic metabolites in biological fluids by high-performance liquid chromatography was developed. Tannic acid and four phenolic compounds, namely gallic acid, pyrogallol, 4-O-methylgallic acid and ellagic acid, were successfully extracted from the biological fluids by using ethyl acetate at acidic conditions. Gallic acid, pyrogallol and 4-O-methylgallic acid were found in the sheep urine, gallic acid. 4-O-methylgallic acid and ellagic acid in plasma, and gallic acid and ellagic acid in abomasal fluid after abomasal dosing of tannic acid. Tannic acid was found in the plasma apart from the abomasal fluid into which it was administered. The concentrations of tannic acid, gallic acid. pyrogallol, 4-O-methylgallic acid and ellagic acid in plasma. abomasal fluid and urine were measured. This method could be applied to measurement of other hydrolysable tannins and their phenolic metabolites in biological materials.

引用

页码：77 / 85

页数：9

共 21 条

[1] DETERMINATION AND CHARACTERIZATION OF GALLOTANNIN BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].