A HUMAN HEART CDNA LIBRARY - THE DEVELOPMENT OF AN EFFICIENT AND SIMPLE METHOD FOR AUTOMATED DNA-SEQUENCING

The Development of an efficient and simple method for automated DNA sequencing. Journal of Molecular and Cellular Cardiology (1993) 25, 891-894. A direct polymerase chain reaction (PCR) amplification of the human heart cDNA clones was used to generate λ PCR product. By the use of the first set of primers derived from λg11, each cDNA insert can readily be obtained. Using millipore filters, primers and nucleotides are removed and this purified PCR product can then be subjected to a second set of fluorescent primers in the generation of nucleotides in the auto-cycle reactions for automated DNA sequencing. © 1993 Academic Press Limited.