CHARACTERIZATION OF THE PYRUVATE KINASE-ENCODING GENE (PKIL) OF TRICHODERMA-REESEI

被引：15

作者：

SCHINDLER, M

MACH, RL

VOLLENHOFER, SK

HODITS, R

GRUBER, F

VISSER, J

DEGRAAFF, L

KUBICEK, CP

机构：

[1] VIENNA TECH UNIV,INST BIOCHEM TECHNOL & MIKROBIOL,MIKROBIELLE BIOCHEM ABT,A-1060 VIENNA,AUSTRIA

[2] AGR UNIV WAGENINGEN,DEPT GENET,MOLEC GENET SECT,6700 HB WAGENINGEN,NETHERLANDS

来源：

GENE | 1993年 / 130卷 / 02期

关键词：

RECOMBINANT DNA; GLYCOLYTIC GENES; ASPERGILLUS; INTRON; FUNGI;

D O I：

10.1016/0378-1119(93)90430-B

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The pyruvate kinase-encoding gene (pkil) from Trichoderma reesei was isolated by hybridization to the corresponding Aspergillus nidulans pkiA gene. The 1614-bp nucleotide (nt) sequence of the cloned gene codes for a 538-amino-acid protein. The coding sequence contains a single intron of 246 nt at a position identical to that of intron E in the A. nidulans gene. The PKI protein shows extensive homology to the PKIs of A. nidulans and A. niger (67%) and Saccharomyces cerevisiae (59%). The 5' non-coding sequence contains a number of motifs typical for yeast glycolytic genes, but so far only rarely found in filamentous fungi.

引用

页码：271 / 275

页数：5

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