MODIFIED TETRAZOLIUM-BASED COLORIMETRIC METHOD FOR DETERMINING THE ACTIVITIES OF ANTI-HIV COMPOUNDS

被引：5

作者：

AYISI, NK

GUPTA, SV

QUALTIERE, LF

机构：

[1] UNIV SASKATCHEWAN,WESTERN COLL VET MED,DEPT PHYSIOL SCI,SASKATOON S7N 0W0,SASKATCHEWAN,CANADA

[2] UNIV SASKATCHEWAN,COLL MED,DEPT MICROBIOL,SASKATOON S7N 0W0,SASKATCHEWAN,CANADA

来源：

JOURNAL OF VIROLOGICAL METHODS | 1991年 / 33卷 / 03期

关键词：

HIV; CHEMOTHERAPY; MTT; PPL; MODIFIED FORMULA;

D O I：

10.1016/0166-0934(91)90033-V

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A modified tetrazolium-based colorimetric assay was used to determine the anti-HIV activities of ddAzThd, ddCyd, ddIno, and PFA. In this assay, poly-1-lysine-coated plates were used to attach the MT-2 cells to the bottom of the plates. A fixed amount of virus (50 TCID50) was used in each well. A modified version of the formula published by Pauwels et al. (1988) was used for calculating the percentage cell protection from virus infection. Using CC10/EC90 EC90 to calculate the selective indices, the decreasing order of selectivity against HIV-1 strain A87SF, was: ddAzThd > PFA > ddCyd > ddIno. Against HIV-1 strain A79SK-1 the decreasing order of selectivity was: PFA > ddIno > AzThd > ddCyd. The modified formula showed lack of anti-HIV activity for thymidine at non-toxic concentrations.

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收藏

页码：335 / 344

页数：10

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