QUANTITATION OF BLOOD-LYMPHOCYTES SECRETING ANTIBODIES TO PNEUMOCOCCAL POLYSACCHARIDES AFTER INVIVO ANTIGENIC-STIMULATION

被引：32

作者：

HEILMANN, C

PEDERSEN, FK

机构：

[1] UNIV COPENHAGEN, DEPT PAEDIAT, DK-2200 COPENHAGEN, DENMARK

[2] STATENS SERUM INST, WHO, COLLABORATING CTR REFERENCE & RES PNEUMOCOCCI, DK-2300 COPENHAGEN, DENMARK

来源：

SCANDINAVIAN JOURNAL OF IMMUNOLOGY | 1986年 / 23卷 / 02期

关键词：

D O I：

10.1111/j.1365-3083.1986.tb01957.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

An indirect plaque-forming cell assay detecting B cells secreting IgM, IgG and IgA antibodies against pneumococcal polysaccharides (PPS) is described. The numbers of anti-PPS-secreting cells (SC) and Ig-SC in the blood of normal persons immunized with a polyvalent PPS vaccine were quantitated. Anti-PPS-SC were recorded from the fourth to the twelfth post-vaccination day, and the maximum number was found between days 6 and 9. Quantitatively IgA anti-PPS-SC outnumbered the IgM and IgG anti-PPS-SC. Concomitant with the increase in the numbers of antibody-SC an increase in polyclonally activated IgM-, IgG- and IgA-SC was recorded. The specific anti-PPS-antibody-SC constituted 20-80% of the total numbers of Ig-SC from the sixth to the ninth post-vaccination day.

引用

页码：189 / 194

页数：6

共 15 条

[1] PRODUCTION AND SECRETION OF IMMUNOGLOBULINS BY INVITRO-ACTIVATED HUMAN LYMPHOCYTES-B [J].