TRIPLE HELIX SPECIFIC LIGANDS

被引：306

作者：

MERGNY, JL

DUVALVALENTIN, G

NGUYEN, CH

PERROUAULT, L

FAUCON, B

ROUGEE, M

MONTENAYGARESTIER, T

BISAGNI, E

HELENE, C

机构：

[1] MUSEUM NATL HIST NAT, BIOPHYS LAB,CNRS,UA 481,INSERM,U201,43 RUE CUVIER, F-75005 PARIS, FRANCE

[2] INST CURIE, SYNTH LAB, CNRS, URA 1387, F-91405 ORSAY, FRANCE

来源：

SCIENCE | 1992年 / 256卷 / 5064期

关键词：

D O I：

10.1126/science.256.5064.1681

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A triple helix is formed upon binding of an oligodeoxynucleotide to the major groove of duplex DNA. A benzo[e]pyridoindole derivative (BePI) strongly stabilized this structure and showed preferential binding to a triplex rather than to a duplex. Energy transfer experiments suggest that BePI intercalates within the triple helix. Sequence-specific inhibition of transcription initiation of a specific gene by Escherichia coli RNA polymerase by a triplex-forming oligodeoxynucleotide is strongly enhanced when the triplex is stabilized by BePI. Upon irradiation with ultraviolet light, BePI induces covalent modifications of the target within the triple helix structure.

引用

收藏

页码：1681 / 1684

页数：4

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