ZONA-PELLUCIDA SURFACE OF IMMATURE AND INVITRO MATURED MOUSE OOCYTES - ANALYSIS BY SCANNING ELECTRON-MICROSCOPY

被引：29

作者：

CALAFELL, JM

NOGUES, C

PONSA, M

SANTALO, J

EGOZCUE, J

机构：

[1] Dept. Biologia Cel.lular i Fisiologia, Unitat Biologia Cel.lular. Fac. Ciències, Universitat Autònoma de Barcelona, Bellaterra Barcelona

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 1992年 / 9卷 / 04期

关键词：

SCANNING ELECTRON MICROSCOPY (SEM); ZONA-PELLUCIDA; OOCYTE INVITRO MATURATION; MORPHOLOGY;

D O I：

10.1007/BF01203961

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Purpose: The aim of this work was to determine the morphology of the zona pellucida surface of immature and in vitro matured mouse oocytes by scanning electron microscopy. For this purpose two groups of immature oocytes (germinal vesicle group and metaphase I group) were studied either before or after in vitro maturation. Results: Before in vitro maturation, the germinal vesicle immature group showed mainly an unstructured zona pellucida surface with smooth cumulus cells. The metaphase I immature group showed a more structured Zona pellucida with smooth or blebbing cumulus cells. After in vitro maturation, development of the zona pellucida toward a mature surface, related to the initial degree of oocyte maturity, was observed in both groups. Conclusions: These observations show a correlation between the morphology of the zona pellucida surface and the degree of oocyte maturity; the in vitro maturation process can give rise to a proper development of this endowment when immature oocytes are used.

引用

页码：365 / 372

页数：8

共 21 条

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