INCREASED FISH EFFICIENCY USING APC PROBES GENERATED BY DIRECT INCORPORATION OF LABELED NUCLEOTIDES BY PCR

被引：43

作者：

RICHARD, F ^{[1
]}

VOGT, N ^{[1
]}

MULERIS, M ^{[1
]}

MALFOY, B ^{[1
]}

DUTRILLAUX, B ^{[1
]}

机构：

[1] INST CURIE,BIOL SECT,CNRS,URA 620,26 RUE ULM,F-75231 PARIS 05,FRANCE

来源：

CYTOGENETICS AND CELL GENETICS | 1994年 / 65卷 / 03期

关键词：

D O I：

10.1159/000133624

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Probes of various sizes from the adenomatous polyposis coli gene (APC) were directly biotinylated by polymerase chain reaction (PCR) from genomic DNA. PCR labeling gave high efficiency in detection of fluorescence in situ hybridization (FISH) signals. Probes as small as 250 base pairs could be visualized through a fluorescence microscope without any image processing.

引用

页码：169 / 171

页数：3

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