STEREOSPECIFIC ACYL TRANSFERS ON THE ERYTHROMYCIN-PRODUCING POLYKETIDE SYNTHASE

被引：150

作者：

MARSDEN, AFA

CAFFREY, P

APARICIO, JF

LOUGHRAN, MS

STAUNTON, J

LEADLAY, PF

机构：

[1] UNIV CAMBRIDGE,CAMBRIDGE CTR MOLEC RECOGNIT,CAMBRIDGE CB2 1QW,ENGLAND

[2] UNIV CAMBRIDGE,DEPT BIOCHEM,CAMBRIDGE CB2 1QW,ENGLAND

[3] UNIV CAMBRIDGE,CAMBRIDGE CTR MOLEC RECOGNIT,CAMBRIDGE CB2 1EW,ENGLAND

[4] UNIV CAMBRIDGE,CHEM LAB,DEPT ORGAN CHEM,CAMBRIDGE CB2 1EW,ENGLAND

来源：

SCIENCE | 1994年 / 263卷 / 5145期

关键词：

D O I：

10.1126/science.8278811

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

During assembly of complex polyketide antibiotics like erythromycin A, molecular recognition by the multienzyme polyketide synthase controls the stereochemical outcome as each successive methylmalonyl-coenzyme A (CoA) extender unit is added. Acylation of the purified erythromycin-producing polyketide synthase has shown that all six acyltransferase domains have identical stereospecificity for their normal substrate (2S)-methylmalonyl-CoA. In contrast, the configuration of the methyl-branched centers in the products, that are derived from (2S)-methylmalonyl-CoA, is different. Stereoselection during the chain building process must, therefore, involve additional epimerization steps.

引用

页码：378 / 380

页数：3

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