A NOVEL CHEMOENZYMATIC GLYCOSYLATION STRATEGY - APPLICATION TO LYSOZYME MODIFICATION

被引：31

作者：

LONGO, MA ^{[1
]}

COMBES, D ^{[1
]}

机构：

[1] INST NATL SCI APPL, CTR BIOINGN GILBERT DURAND, CNRS, URA 544, F-31077 TOULOUSE, FRANCE

来源：

FEBS LETTERS | 1995年 / 375卷 / 1-2期

关键词：

GLYCOSYLATION; LYSOZYME; GLYCOSYLTRANSFERASE; HYDROPHOBICITY; STABILITY;

D O I：

10.1016/0014-5793(95)01174-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hen egg lysozyme has been non-specifically glycosylated using a novel two-step strategy, First, a number of sucrose molecules have been chemically bound to the protein surface lysines, then the glycosidic chains have been enzymically lengthened, using a glycosyltransferase. For this task, a fructosyltransferase and a levansucrase have been tested, the latter appearing as the most effective one, In all cases, reactions have been optimised and several degrees of modification have been obtained, Finally, the effects of the modifications on lysozyme hydrophobicity, hydrolytic activity, hydrolysis substrate affinity and thermostability have been assessed.

引用

页码：63 / 66

页数：4

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