THE RAT-BRAIN SYNUCLEINS - FAMILY OF PROTEINS TRANSIENTLY ASSOCIATED WITH NEURONAL MEMBRANE

被引：298

作者：

MAROTEAUX, L ^{[1
]}

SCHELLER, RH ^{[1
]}

机构：

[1] STANFORD UNIV,HOWARD HUGHES MED INST,DEPT MOLEC & CELLULAR PHYSIOL,STANFORD,CA 94305

来源：

MOLECULAR BRAIN RESEARCH | 1991年 / 11卷 / 3-4期

关键词：

PHOSPHOINOSITOL 2ND MESSENGER; INSITU HYBRIDIZATION; CHOLINEACETYLTRANSFERASE; CDNA CLONING; IMMUNOCYTOCHEMISTRY;

D O I：

10.1016/0169-328X(91)90043-W

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The synuclein cDNA was previously cloned from Torpedo califonica using an antiserum against purified synaptic vesicles. Here we show that this gene hybridizes to several clones in a rat brain cDNA library. These clones represent cDNAs coding for different proteins, thus determining a family. These proteins display similar organization to the Torpedo protein. The homology resides within the repetition of 7 amino acids. The diversity is generated by alternative splicing as suggested by Southern analysis, as well as by sequence analysis. These proteins are specifically expressed in the rat brain. In situ hybridization shows expression of synuclein mRNAs in discrete areas of the brain, the hippocampus is the most intensely stained area. This localization is reminiscent of those molecules involved in phosphoinositols second messenger pathways. Synuclein proteins are present in rat brain in dual form - a small form which is soluble and a large form which is associated with synaptosomal membranes in a regulated fashion.

引用

页码：335 / 343

页数：9

共 30 条

[1]

Bayer S.A, 1985, RAT NERVOUS SYSTEM, V1, P335

[2] DISTINCT PATTERNS OF EXPRESSION OF DIFFERENT PROTEIN-KINASE-C MESSENGER-RNAS IN RAT-TISSUES [J].

BRANDT, SJ ;

NIEDEL, JE ;

BELL, RM ;

YOUNG, WS .

CELL, 1987, 49 (01) :57-63

[3]

BUCKLEY NJ, 1988, J NEUROSCI, V8, P4646

[4]

CHAN SY, 1986, J NEUROSCI, V6, P3618

[5] EUKARYOTIC PROTEIN MODIFICATION AND MEMBRANE ATTACHMENT VIA PHOSPHATIDYLINOSITOL [J].

CROSS, GAM .

CELL, 1987, 48 (02) :179-181

[6] PRIMARY STRUCTURE AND EXPRESSION OF BETA-2 - A NOVEL SUBUNIT OF NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTORS [J].

DENERIS, ES ;

CONNOLLY, J ;

BOULTER, J ;

WADA, E ;

WADA, K ;

SWANSON, LW ;

PATRICK, J ;

HEINEMANN, S .

NEURON, 1988, 1 (01) :45-54

[7] PROTEIN IMPORT INTO THE CELL-NUCLEUS [J].

DINGWALL, C ;

LASKEY, RA .

ANNUAL REVIEW OF CELL BIOLOGY, 1986, 2 :367-390

[8]

FERGUSON MAJ, 1988, ANNU REV BIOCHEM, V57, P285

[9] MULTIPLE NEUROPEPTIDES DERIVED FROM A COMMON PRECURSOR ARE DIFFERENTIALLY PACKAGED AND TRANSPORTED [J].

FISHER, JM ;

SOSSIN, W ;

NEWCOMB, R ;

SCHELLER, RH .

CELL, 1988, 54 (06) :813-822

[10]

FUQUA SA, 1989, CANCER RES, V49, P4127

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