PRODUCTION OF CELL-BOUND PROTEINASE BY LACTOBACILLUS-BULGARICUS AND ITS LOCATION IN BACTERIAL-CELL

被引：57

作者：

ARGYLE, PJ

MATHISON, GE

CHANDAN, RC

机构：

[1] QUEEN ELIZABETH COLL, DEPT MICROBIOL, LONDON W8 7AH, ENGLAND

[2] UNILEVER RES, COLWORTH WELWYN LAB, WELWYN AL6 9AG, HERTFORDSHIRE, ENGLAND

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1976年 / 41卷 / 01期

关键词：

D O I：

10.1111/j.1365-2672.1976.tb00616.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

L. bulgaricus NCDO 1489 produced a single, cell-bound proteinase during growth on nutrient medium at 45.degree. C. Proteinase activity was optimal at 45-50.degree. C and pH 5.2-5.8, and was inhibited by chelating agents [EDTA, sodium citrate]. The enzyme was mainly associated with the cell envelope but was liberated from cells under conditions favoring autolysis or by treatment of the cells with lysozyme. Its relation to the growth of the organism in milk and possible role in formation of fermented milk products are discussed.

引用

页码：175 / 184

页数：10

共 16 条

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