A MONOCLONAL-ANTIBODY SANDWICH IMMUNOASSAY FOR SERUM AMYLOID-A (SAA) PROTEIN

被引：79

作者：

MCDONALD, TL

WEBER, A

SMITH, JW

机构：

[1] Department of Pathology and microbiology, University of Nebraska Medical Center, Omaha

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1991年 / 144卷 / 02期

关键词：

SERUM AMYLOID-A; C-REACTIVE PROTEIN; ELISA; MONOCLONAL ANTIBODY;

D O I：

10.1016/0022-1759(91)90081-P

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An antibody sandwich immunoassay using two purified rat monoclonal antibodies to human serum amyloid A was developed and used to measure serum amyloid A in human serum. The assay was specific, sensitive, reproducible, and reliable and does not require denaturation of the specimen prior to assay. Serum amyloid A purified by hydrophobic interaction chromatography of acute phase human serum afforded a reliable standard for the assay. A significant (r = 0.69) correlation for SAA and C reactive protein values was found for 180 patient samples analyzed.

引用

页码：149 / 155

页数：7

共 32 条

[1]

BENDITT EP, 1988, METHOD ENZYMOL, V163, P510

[2] SERUM AMYLOID A PROTEIN IN AMYLOIDOSIS, RHEUMATIC, AND NEOPLASTIC DISEASES [J].