INDUCTION OF IMMEDIATE EARLY AND STRESS GENES IN RAT PROXIMAL TUBULE EPITHELIUM FOLLOWING INJURY - THE SIGNIFICANCE OF CYTOSOLIC IONIZED CALCIUM

被引：20

作者：

YAMAMOTO, N

MAKI, A

SWANN, JD

BEREZESKY, IK

TRUMP, BF

机构：

[1] Department of Pathology, University of Maryland School of Medicine, Baltimore, MD

[2] Maryland Institute for Emergency Medical Services System, Baltimore, MD

来源：

RENAL FAILURE | 1993年 / 15卷 / 02期

关键词：

D O I：

10.3109/08860229309046148

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

This study was designed to investigate the influence of intracellular ionized calcium ([Ca2+]i) on the induction of c-fos, c-jun, c-myc, and hsp70 genes after oxidant stress induced by xanthine/xanthine oxidase (X/XOD) treatment or after heat shock using primary cultures of rat proximal tubule epithelium (PTE). X/XOD (500 muM/25 mU/mL) induced all of these genes; ionomycin also resulted in similar kinetics of induction of all genes. The expression of both c-fos following X/XOD treatment and hsp70 following heat shock was markedly decreased through chelation of [Ca2+]i by Quin 2/AM. The c-fos expression following X/XOD treatment was partly reduced by a protein kinase C inhibitor, staurosporine (ST), and markedly inhibited by another protein kinase inhibitor, 2-aminopurine (2AP), while both ST and 2AP markedly reduced hsp70 expression. The ADP-ribosylation transferase inhibitor 3-aminobenzamide had no effect on either c-fos or hsp70 expression. These results suggest that cell injuries leading to increased [Ca2+]i in PTE result in induction of c-fos, c-jun, c-myc, and hsp70; and that the activation of c-fos and hsp70 genes may be regulated by [Ca2+]i and [Ca2+]i-dependent protein kinases.

引用

页码：163 / 171

页数：9

共 48 条

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