PURIFICATION OF A 57KDA NUCLEAR MATRIX PROTEIN ASSOCIATED WITH THIOL-PROTEIN-DISULFIDE OXIDOREDUCTASE AND PHOSPHOLIPASE-C ACTIVITIES

被引：37

作者：

ALTIERI, F ^{[1
]}

MARAS, B ^{[1
]}

EUFEMI, M ^{[1
]}

FERRARO, A ^{[1
]}

TURANO, C ^{[1
]}

机构：

[1] UNIV ROMA LA SAPIENZA,CNR,CTR MOLEC BIOL,I-00185 ROME,ITALY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 194卷 / 03期

关键词：

D O I：

10.1006/bbrc.1993.1919

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteins of the internal nuclear matrix from chicken liver were fractionated, by chromatographic procedures, in non denaturing conditions. At least two fractions were present with phosphatidylinositol-specific phospholipase C and three with thiol:protein-disulflde oxidoreductase activity. A 57kDa protein was isolated which copurified with both these activities. Partial amino acid sequences showed a high degree of homology with a cytosolic protein previously identified as a phospholipase C and with a microsomal protein identified as a thiol:protein-disulfide oxidoreductase. Our finding leaves the question still unanswered of the real function of this protein, which for the first time has been isolated from the nuclear matrix. © 1993 Academic Press. All rights reserved.

引用

页码：992 / 1000

页数：9

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