IMMOBILIZATION OF PERALKYLATED BETA-CYCLODEXTRIN ON SILICA-GEL FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：39

作者：

CIUCANU, I ^{[1
]}

KONIG, WA ^{[1
]}

机构：

[1] UNIV HAMBURG,INST ORGAN CHEM,D-20146 HAMBURG,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 685卷 / 01期

关键词：

D O I：

10.1016/0021-9673(94)00564-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The synthesis of mono-O-5-pent-1-enyl-beta-cyclodextrin was studied in order to obtain the best yield of mono-O-substitution. Permethylated mono-O-5-pent-1-enyl-beta-cyclodextrin, purified by liquid chromatography, was characterized by fast atom bombardment MS and H-1 NMR spectrometry. GC-MS analysis of partially methylated alditol acetates obtained from mono-O-pent-1-enyl-beta-cyclodextrin showed that the 5-pent-1-enyl ether group was in the cu-D-glucopyranoside residue in the 2-OH position in a proportion of 95.9% for the O-substitution reaction in dimethyl sulfoxide and in the presence of sodium hydroxide. Silica gel with chemically bonded peralkylated(methyl or propyl)-beta-cyclodextrin proved to be an efficient stationary phase for the separation of enantiomers.

引用

页码：166 / 171

页数：6

共 18 条

[1]

Armstrong D. W., 1985, US Pat., Patent No. [4 539 399, 4539399, 4,539,399]

[2]

BJOERNDAL H, 1970, ANGEW CHEM, V16, P643

[3]

BRENDSEN GE, 1978, J LIQ CHROMATOGR, V1, P561

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