HOUSE DUST MITE-DERIVED AMYLASE - ALLERGENICITY AND PHYSICOCHEMICAL CHARACTERIZATION

被引:80
作者
LAKE, FR
WARD, LD
SIMPSON, RJ
THOMPSON, PJ
STEWART, GA
机构
[1] PRINCESS MARGARET HOSP,WESTERN AUSTRALIAN RES INST CHILD HLTH,THOMAS ST,PERTH,WA 6008,AUSTRALIA
[2] UNIV WESTERN AUSTRALIA,DEPT MED,PERTH,WA,AUSTRALIA
[3] ROYAL MELBOURNE HOSP,WALTER & ELIZA HALL INST MED RES,LUDWIG INST CANC RES,JOINT PROT STRUCT LAB,PARKVILLE,VIC 3050,AUSTRALIA
基金
英国医学研究理事会;
关键词
D O I
10.1016/0091-6749(91)92147-S
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Amylase activity was found in extracts of both Dermatophagoides pteronyssinus whole mite (0.16 U/mg) and spent growth medium (0.01 U/mg) but not in unused growth medium. It was also detected in all extracts of house dust obtained from mattresses (n = 20; geometric mean, 1.95 U/gm) and in 18 extracts of dust obtained from lounge room carpets (n = 20; geometric mean, 0.54 U/gm). Although the origins of amylase in dust are unclear, enzyme activity correlated with mite counts (n = 40; r = 0.35; p < 0.05) and Der p I concentrations (r = 0.41; p < 0.01). Mite amylase was purified from spent growth medium by affinity chromatography, gel filtration, and chromatofocusing. It was physicochemically similar to mammalian amylase with regard to molecular weight (60,000), charge heterogeneity (isoelectric point, 5 to 7) and the capacity to bind to an organomercurial affinity matrix. The optimum pH for enzymatic activity was revealed to be 6.4. IgE immunoblot studies demonstrated that the enzyme was allergenic and that its expression was dependent on the integrity of intrachain disulfide bonds. Sera from 25% of mite-allergic children and 46% of mite-allergic adults contained specific IgE to mite amylase. IgE to amylase was associated (p < 0.01) with increased concentrations of total mite-specific IgE determined with a direct RAST assay.
引用
收藏
页码:1035 / 1042
页数:8
相关论文
共 33 条
[1]  
Baldo B.A., 1989, Advances in the Biosciences, V74, P13
[2]   ENZYMES ARE THE COMPONENTS OF INHALED PANCREATIN EFFECTIVE AS ALLERGENS [J].
BAUR, X ;
WIESSMANN, KJ ;
WUTHRICH, B .
DEUTSCHE MEDIZINISCHE WOCHENSCHRIFT, 1984, 109 (07) :257-260
[3]  
BAUR X, 1986, LANCET, V1, P43
[4]  
BEGG GS, 1989, METHODS PROTEIN SEQU, P108
[5]   AMYLASE AND ESTERASE POLYMORPHISMS IN ECONOMICALLY IMPORTANT STORED PRODUCT MITES (ACARI, ASTIGMATA) [J].
BOWMAN, CE ;
LESSITER, MJ .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1985, 81 (02) :353-360
[6]   POLYSACCHARIDASES IN ASTIGMATID MITES (ARTHROPODA, ACARI) [J].
BOWMAN, CE ;
CHILDS, M .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1982, 72 (04) :551-557
[7]  
BOWMAN CE, 1984, ACAROLOGY 6, V2, P993
[8]   ISOLATION OF CDNA CODING FOR THE MAJOR MITE ALLERGEN DER P-II BY IGE PLAQUE IMMUNOASSAY [J].
CHUA, KY ;
DOYLE, CR ;
SIMPSON, RJ ;
TURNER, KJ ;
STEWART, GA ;
THOMAS, WR .
INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1990, 91 (02) :118-123
[9]   SEQUENCE-ANALYSIS OF CDNA CODING FOR A MAJOR HOUSE DUST MITE ALLERGEN, DER-P-1 HOMOLOGY WITH CYSTEINE PROTEASES [J].
CHUA, KY ;
STEWART, GA ;
THOMAS, WR ;
SIMPSON, RJ ;
DILWORTH, RJ ;
PLOZZA, TM ;
TURNER, KJ .
JOURNAL OF EXPERIMENTAL MEDICINE, 1988, 167 (01) :175-182
[10]  
COLLOFF MJ, IN PRESS CLIN EXP AL