BIOSYNTHESIS OF THE THIAZOLE MOIETY OF THIAMIN (VITAMIN-B1) IN HIGHER-PLANT CHLOROPLASTS

被引：110

作者：

JULLIARD, JH ^{[1
]}

DOUCE, R ^{[1
]}

机构：

[1] RHONE POULENC AGROCHIM,CTR RECH DARGOIRE,CNRS,UNITE MIXTE 41,14-20 IMPASSE P BAIZET,F-69263 LYONS,FRANCE

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 06期

关键词：

CHLOROPLAST; 1-DEOXY-D-THREO-2-PENTULOSE; SPINACIA-OLERACEA;

D O I：

10.1073/pnas.88.6.2042

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The chloroplast stromal proteins from spinach condense the two heterocyclic moieties of vitamin B1-4-methyl-5-(beta-hydroxyethyl)thiazole and 2-methyl-4-amino-5-hydroxymethylpyrimidine-in the presence of MgATP. We have taken advantage of this observation to study thiazole synthesis. We present data indicating that pyruvate, glyceraldehyde 3-phosphate, tyrosine, cysteine, and MgATP are required for this synthesis and that pyruvate and glyceraldehyde 3-phosphate can be replaced by 1-deoxy-D-threo-2-pentulose. The thiazole synthesis occurs at a sustained, low rate of 140 pmol per mg of protein per hr.

引用

页码：2042 / 2045

页数：4

共 28 条

[1]

[Anonymous], METHODS CHLOROPLAST

[2] ORIGIN OF SULFUR ATOM IN THIAMINE [J].